Absorption Spectrum Analysis of Dentine Sialophosphoprotein (DSPP) in Orthodoctic Patient

150 150 MIMOS Berhad


Mohd Norzaliman Mohd Zain, Zalhan Md Yusof, Farinawati Y. , Asma A., Ken, WSH, Lee, WJ , Tan, KF, Shahrul , HA and Rohaya



Aforce applied during the orthodontic treatment induces inflammation which is essential for tooth movement, can leadtoroot resorption. Previous research works haveproved aprotein biomarker which can monitorthe root resorption during orthodontic tooth movement. Dentine sialophosphoprotein (DSPP) is one of most abundant non-collagenous protein in dentine. DSPP is released into Gingival crevicular fluid (GCF)during external root resorption. In this work, we investigate and analysis the absorbance spectrum of human DSPP by using spectroscopyand a qualitative model using Soft Independent Modelling Class Analogies (SIMCA). The absorption spectrum data will be used asan indicatorfor clinical examination of root resorption in orthodontic treatment. The subjects for this study consisted of non-orthodonticand orthodontic patientsbased on different clinical treatment period. GCF samples collected from both non-orthodontic and orthodontic groups showed ultra-violet spectrum range from 244.11 to 259.86 nm. The spectrum data model accuracy for non-orthodontic and orthodontic patient obtained at 0.91. The result indicates that GCFabsorption spectrumsobtained correlated with the duration of orthodontic treatmentafter the occurrence of Orthodontic-induced inflammatory root resorption(OIIRR). The qualitative spectrum data model developed is capable to classify samples into non-orthodontic and orthodontic groups.



2nd International Conference on Applied Photonics and Electronics (InCAPE 2019), Putrajaya